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Objective:To understand the clinical manifestations, diagnosis, treatment, and prognosis of children with hereditary thrombocytopenia (HT).Methods:The clinical data of 5 patients with HT in the Hematology and Oncology Department of Children′s Hospital of Chongqing Medical University from August 2015 to October 2017 were retrospectively analyzed. The clinical and laboratory characteristics, treatment, and prognosis of HT were discussed by reviewing relevant literatures.Results:Five patients included 3 boys and 2 girls.The median age at onset of 4 years and 2 months old and the median age at diagnose was 4 years and 4 months old.All patients presented with the thrombocytopenia, among which 4 cases were macrothrombocytopenia and 1 case was normothrombocytopenia.The main clinical presentations of 5 patients were skin petechiae and ecchymoses.Four cases were initially misdiagnosed as immune thrombocytopenia (ITP) and received the glucocorticoid and immunoglobulin, while the therapeutic effect was not satisfactory.The gene sequencing confirmed MYH9 gene mutation(c.3493C>T), MYH9 gene mutation(c.5878G>A), NBEAL2 gene compound heterozygous mutation(c.295C>T; c.4169C>T), GP1BA gene mutation(c.1761A>C), and ANKRD26 gene mutation(c.5123A>G), in 5 patients respectively. Conclusions:HT should be suspected among those with recurrent isolated thrombocytopenia and no response to the ITP regimen, and the early gene screening is of great significance to the patients′ treatment and prognosis.
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Objectives:This study aims to provide a full picture of how Health Policy and Systems Research ( HPSR) in Chinese universities. Based on the current situation, analysis of problems and challenges of the HPSR capacity has been conducted and suggestions on personnel training have been proposed. Methods: Quantitative sur-veys and qualitative interviews have been conducted among personnel engaged in HPSR from 8 representative univer-sities. Results:Most HPSR researchers in the universities are young, of which 75. 26% have doctorates and the ma-jority is trained in health care management and public health sciences. Among the current HPSR projects, health in-formation and evidence is the main research direction in this field, and both the capital investment and attention of re-searchers are high. However, research resources and funding are still currently focused on senior researchers, with limited efforts to support young scholars. HPSR has interdisciplinary and multidisciplinary characteristics, but cur-rently the collaboration among institutions and individuals is relatively limited. In addition, only 5. 81% of undertak-en HPSR projects have been translated into health policy. Discussion and Suggestions:HPSR researchers in Chinese universities are not sufficient and have similar backgrounds, resulting in limited collaboration. Investment in HPSR projects is still shallow and young researchers are vulnerable in acquiring funding resources. The ability to translate research results into policy is weak. Therefore, this paper suggests to mainstream undergraduate and postgraduate teaching, enhance the training, funding and technical support for young researchers, and to establish the mechanism of dissemination of research findings and their translation into policy.
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Objective To investigate the status quo of the assurance and subsidy for severe diseases acquired by juvenile in rural areas in Qinghai, and provide evidences for building a subsidy system for such population. Methods Quantitative surveys were made to collect data on medical expenses and the compensation practice for such expenses for severe diseases of juvenile in the area, for a knowledge of the present medication and disease load in question; specialists in two hospitals were interviewed to learn the present treatnents for such a population, and their expenditures in case of such diseases. Results Juveniles in rural Qinghai rely on the new rural cooperative medical scheme, under which however the reimbursement rate was only 26% ～35%. Families with juvenile patients of severe diseases were found to suffer heavy burdens. As a result, a high percentage of the patients give up treatment. To make things worse, as only two hospitals in the provincial capital city can provide professional treatment for children with severe diseases, rural children have to pay heavy indirect costs other than medication such as heavy travel expenses. Conclusion It is critical to build a medical assurance system for juvenile with severe diseases. It is also imperative to build and strengthen the capacity of medical centers for such a population in Qinghai, and upgrade the diagnosis and treatment competence of local health providers, ensuring adequate medical resources for juvenile with severe diseases upon joint development of both healthcare providers and the medical insurance system.
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ObjectiveTo investigate the inhibitory effect of 1-methyl-tryptophan (1-MT) on transplanted hepatocellular carcinoma in mice.MethodsHuman hepatocellular carcinoma subcutaneous tumor models were established in mice,and the mice were divided into hepG2 group,empty plasmid group,indoleamine 2,3-dioxygenase (IDO) saline group,IDO) 5-fluoropyrimidine (5-FU) group,IDO 1-MT group,and the group combining IDO 1-MT with 5-FU treatment (n=8 in each group).The tumor growth,tumor volume and pathological examination were observed and the expression of IDO in tumor tissues was determined by immunohistochemistry.ResultsCompared with hepG2 and the empty vector saline groups,IDO saline group had bigger tumor,faster growth,and the differences were statistically significant (P＜0.05).Compared with IDO saline group,5-FU group,1-MT group and combination treatment groups showed smaller tumor volume and weight,and the tumor inhibitory rates were 86.54％,79.95％,94.46％,respectively.There were significant differences between these groups (P＜0.05).However,there were no significant differences in tumor volumes between 5-FU group and 1-MT group (P＞0.05).HE pathological observation of ceils in each treatment group showed reduced density,increased necrotic area and significant decrease in peripheral blood alpha-fetoprotein (P＜0.05).ConclusionsIDO can promote the growth of liver cancer cells involved in immune escape.1-MT can inhibit the transplanted tumor growth in mice,and therefore may enhance the chemotherapeutic efficacy.
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@#Objective To investigate the total number, reasons, and distributing characteristics of vagrant psychosis patients in Xi'an. Methods The colony random sample in 4 objective research units from 2005 to 2010 in Xi'an, Shaanxi were included. Databank was set up according to the total number of vagrant psychosis patients based on basic information. Grading statistics was used. Results Total number of vagrant psychosis patients and salvation needs increased year by year in Xi'an. Home return and social assistance were the main approaches for distributing characteristics of medical assistance. Conclusion The total number of vagrant psychosis patients is relative large. Such number is increasing year by year in Xi'an. A better treatment and diagnostic system for vagrant psychosis patients should be set up governmentally as quickly as possible.
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Artificial antigen Cd-IEDTA-BSA (HSA) was successfully synthesized by the bifunctional chelating agent IEDTA (isothiocyanobenzylethyl enediamine tetraacetic acid) coupling with cadmium and protein carrier. The fluorescence characteristics of antigen were determined by fluorescence spectrophotometry and the coupling ratio 11∶ 1-16∶ 1 was obtained for the coupling reaction. The folding status of serum albumin during the synthesis process of artificial antigen Cd-IEDTA-BSA (HSA) was analyzed using "fluorescence phase diagram", and the result shows that it was conformed to the "all-or-none" pattern. The polarization fluorescence spectra show that the microenvironments of tryptophan residues in carrier protein have some changes to cause changes in protein conformation.
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Objective To explore the discrepancy of anxiety and depression between breast cancer patients and general population,to provide reference for psychological ethical intervention.Methods Breast cancer patients and general population admitted randomly from October.2008 to April.2009 were assessed according to Self-reporting inventory,Self-Rating Depression Scale(SDS)and Self-Rating Anxiety Scale(SAS).Patients with breast cancer were come into the study group(n=100) and general population come into the control group(n=100),and then psychological ethical intervention was provided to each group.Results The Morbidity of anxiety and depression in breast cancer patients is much higher than that in the general population(P